Mukherjee Lab

 

The underlying technique our lab is built upon is a hematopoetic/mesenchymal stem cell coculture. In this system we are able to acurately recreate the bone marrow niche in-vitro, manipulate the microenvironment, and investigate the effects of such manipulations on the niche using fluorescence imaging, flow cytometry, in situ immunohistochemistry, and serial transplants.

co-culture

Our murine leukemic model of AML uses a fluorescent (dsRed) variant of the well documented multipotent, self renewing, hematapoetic cell overexpressing MLL-AF9.

mllaf9

Using shRNAi we have individually knocked down thousands of genes in leukemic and healthy hematapoetic cells. By means of this high-throughput screening, we are discovering the function of many new and exciting genes specifically those involved in quiesence, cell cycle, and differentiation.

screen